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Biological particles can strongly influence the effects of aerosols on atmospheric chemistry and physics, climate, and public health. So far, however, the abundance, diversity, sources, properties, and effects of biological particles in the atmosphere have not yet been well characterized and quantified. This is largely due to a lack of efficient measurement methods. In most earlier studies microscopy, protein staining, or the cultivation of viable airborne microorganisms have been applied. These methods, however, do not allow a comprehensive characterisation of the origin of biological materials independent of particle size, integrity, and viability. In contrast, the application of molecular genetic tools for the analysis of DNA offers a very straightforward way to identify the origin of biological matter from both living and dead, complete and fragmented organisms. Moreover, DNA analysis in combination with additional molecular methods also provides the possibility of estimating the abundance and diversity of different species. In our research project we therefore analyse the biological particles from various regions world wide with molecular genetic tools to identify their diversity, abundance and taxonomic affiliation.